Smartphone-based saliva test that can detect COVID-19 earns UF professor honors in national contest

Dr. Dinglasan

Dr. Rhoel Dinglasan is shown in his laboratory at UF’s Emerging Pathogens Institute. 

By Sarah Carey

A University of Florida professor and his industry collaborators have won second place in a technology competition sponsored by the National Institutes of Health for a smartphone-based, rapid saliva test they developed that can be used to diagnose COVID-19, along with two other diseases of major global importance, malaria and anemia.

A team consisting of Dr. Rhoel Dinglasan, a professor of infectious diseases with UF’s College of Veterinary Medicine and director of the Centers for Disease Control and Prevention’s Southeastern Center of Excellence in Vector Borne Diseases, and two others was one of six winners of the NIH Technology Accelerator Challenge, or NTAC, offered for the first time this year.

The competition was held to encourage the design and development of innovative diagnostic platforms for assessing two major vascular diseases, one of which had to include sickle cell disease, malaria or anemia. Other criteria included accessibility, low cost and the use of a mobile device or a portable attachment to the device.

Dinglasan’s team will receive $200,000 for their entry in the competition, which offered a total of $1 million in prizes to the winners.

“We already had a test we developed for malaria, but this test will go further,” said Dinglasan, the technology’s co-inventor. “This test will leverage existing technology to fill a pressing and unmet need in global public health by allowing us to differentiate between three diseases that can present similarly in terms of symptoms. As treatment and containment protocols differ for each disease, the proper diagnosis delivered in literally minutes could save lives.”

Dinglasan, who also is associated with UF’s Emerging Pathogens Institute, said he was approached earlier this year by Luminostics, a start-up company based in California that was interested in the competition. Company CEO Bala Raja, Ph.D., reached out to Dinglasan in March to collaborate on the challenge submission after seeing a paper in Science Translational Medicine in which Dinglasan’s team reported on their saliva-based rapid diagnostic test for malaria.

“I thought there would be great synergy between his group’s biomarker discovery skills and our platform for rapid diagnostics,” Raja said.

Technology graphic

The CLIP-CAM technology for the Luminostics-UF team involves the collection of saliva by a collection pad on a “lollipop” stick that is set below and to the side of the tongue for 3-5 minutes until the indicator spot changes color, indicating the pad is full of saliva. The pad then is inserted into a syringe, and pushed down to release the saliva into a tube containing stabilizers. The CLIP cassette is affixed to the smartphone, and the corresponding “disease” cassette is inserted and the saliva is added to a port. The sample is then read by an app on the phone that uses the LED flash to initiate detection of SARS-CoV-2 virus antigen, anti-SARS-CoV-2 antibodies, or anemia and malaria parasite biomarkers. (Courtesy of Dr. Bala Raja and Luminostics)

With their skills combined, the team came up with this creative solution to the challenge: the CLIP-CAM, an adapter attached to a smartphone that allows for a detection cassette to be inserted into a slot. The camera flash is used to excite the detection system in the cassette, which can then be read by a smartphone app. Results are available in 15 minutes or less, Dinglasan said.

“We will leverage our leading-edge CLIP platform for the sensitive detection of novel as well as established protein and serological salivary biomarkers for malaria, anemia and COVID-19,” Dinglasan said. Anyone can administer the test to themselves, he said, meaning there is no laboratory required, and no health care worker is needed at any point in the process.

“It’s kind of a selfie for your health, isn’t it?” Dinglasan said. “It’s self-collected; you do it for yourself and your health.”

Although the competition was created well before SARS-CoV-2 infections and COVID-19 disease became prevalent early in 2020, Dinglasan said the technology targeted not only two of the well-established diseases noted in the criteria, but also the “new kid on the block,” COVID-19, which presents with symptoms very similar to the other ailments — and for which a test that can be delivered at the point of care, with no laboratory required, is desperately needed.

“This challenge was written to address blood-borne diseases that have plagued public health and are endemic in many parts of the globe,” Dinglasan said. “But when a person comes into a clinic, what are their symptoms? Fever, malaise, vomiting — what we often refer to as acute febrile illness. When a disease is not malaria or anemia, it could actually be COVID-19. We pushed the envelope; our test provides the ability to assess both what a disease is not, and what it actually is.”

The challenge is supported by the Bill and Melinda Gates Foundation, which will separately review the winners and honorable mentions selected by NIH for potential follow-on funding of up to $500,000 and in-kind support that can transform design concepts into products for global health on an accelerated timeframe.

“We’re looking forward to working with Rhoel’s group on developing a saliva multiplex rapid diagnostic test for COVID-19, malaria and anemia,” Raja said.

Share this article with others:
  • E-mail this story to a friend!
  • Facebook
  • Digg
  • Twitter

August-September 2020

Dr. Dinglasan

Smartphone-based saliva test that can detect COVID-19 earns UF professor honors in national contest

The test developed by Dr. Rhoel Dinglasan and his Luminostics collaborators can also be used to diagnose malaria and anemia.

Cello with animal family members

Goldendoodle survives rare tumor after treatment by UF veterinarians

Veterinarians said the dog could have had only weeks to live without intervention.

The AAA rescue team

Faculty members help find lost dog belonging to family of retired UF CVM professor

Thanks to a network of dog lovers, including two current UFCVM faculty members, a lab mix missing nearly two weeks was found and returned to his grateful owners.

Sea turtle in Cedar Key

Aquatic Animal Health adds new graduate certificate

The college’s Aquatic Animal Health program has added to its educational offerings with a new graduate certificate.

Dr. David Pascual

Professor named associate dean for research and graduate studies

Dr. David Pascual previously fulfilled the administrative role on an interim basis. His appointment is effective immediately.

Alligator Bob

Gators helping Gators: Bob, a 660-pound alligator treated at UF, goes viral

Radiographs taken at UF indicate the 38-year-old alligator has a bone infection, in his right hind leg. He is currently being treated with antibiotics.

Dr. Andrew Allison

Virologist gets grant to study deadly virus that affects conservation animals

A two-year grant from the Morris Animal Foundation will support UFCVM faculty member’s research into an often fatal virus that affects elephants and other key conservation species.

Audrey Kelleman and "Audrey"

College receives funding to create new residency in theriogenology

The AKC Canine Health Foundation will support a new theriogenology residency at UFCVM.

Dr. Schaer

Couple creates professorship honoring UF veterinary college faculty member

The Michael Schaer Professorship in Small Animal Emergency and Critical Care Medicine is the first professorship established at the college dedicated to the support of a clinical faculty member.

Dr. Zhou and Joe Dean talking

Researchers: Molecular “address system” may aid gut health

A molecular address system regulated by a key protein may help further treatment for inflammatory bowel disease and other gut disorders.

...also in this issue



Around the College